The SARS coronavirus nucleocapsid protein – Forms and functions
نویسندگان
چکیده
منابع مشابه
Modular organization of SARS coronavirus nucleocapsid protein.
The SARS-CoV nucleocapsid (N) protein is a major antigen in severe acute respiratory syndrome. It binds to the viral RNA genome and forms the ribonucleoprotein core. The SARS-CoV N protein has also been suggested to be involved in other important functions in the viral life cycle. Here we show that the N protein consists of two non-interacting structural domains, the N-terminal RNA-binding doma...
متن کاملRecombinant SARS coronavirus nucleocapsid protein forms a dimer through its C-terminal domain
Running Title: Mapping the dimerization domain of SARS-CoV N protein Department of Biological Sciences and the Cancer Center, † Bindley Biosciences Center, Purdue University, West Lafayette, IN 47907 State Key Lab for Biocontrol, Zhongshan University, Guangzhou, P.R.China To whom correspondence should be addressed: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1...
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We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healt...
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Severe acute respiratory syndrome (SARS) is a serious infectious disease caused by the SARS coronavirus. We assessed the potential of prime-boost vaccination protocols based on the nucleocapsid (NC) protein co-administered with a derivative of the mucosal adjuvant MALP-2 or expressed by modified Vaccinia virus Ankara (MVA-NC) to stimulate humoral and cellular immune responses at systemic and mu...
متن کاملRecombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein forms a dimer through its C-terminal domain.
The causative agent of severe acute respiratory syndrome (SARS) is the SARS-associated coronavirus, SARS-CoV. The viral nucleocapsid (N) protein plays an essential role in viral RNA packaging. In this study, recombinant SARS-CoV N protein was shown to be dimeric by analytical ultracentrifugation, size exclusion chromatography coupled with light scattering, and chemical cross-linking. Dimeric N ...
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ژورنال
عنوان ژورنال: Antiviral Research
سال: 2014
ISSN: 0166-3542
DOI: 10.1016/j.antiviral.2013.12.009